E-ISSN 2537-0596 | ISSN 2537-0588
 

Original Research 


Apoptotic effects of the medicinal plants Achillea santolina and Raphanus sativus extracts on different cancer cell lines

Mohamed Labib Salem, Reda Mohamed Gafaar, Reham Nagy Mohasseb, Mohamed Ali Abd-Elbaseer.

Abstract
The extracts of the medicinal plants Achillea santolina and Raphanus sativus have been reported to show anti-cancer effects in vitro. However, the cellular and molecular mechanism of these effects are not clear yet. To compare the apoptotic effects of these plant extracts on different cancer cell lines in vitro. The phenolic, flavonoids and antioxidant activity were determined in the crude extracts. Then, Caco2 (colon adenocarcinoma), HepG2 (hepatic carcinoma), MCF7 (breast cancer) and the normal WISH (amniotic cell line) were treated in vitro with different concentrations of crude extracts for 72 hours. The half maximal inhibitory concentration (IC50) were detected by MTT assay, while cell cycle and apoptosis were assessed by flow cytometry. The methanolic extract of R. sativus seeds (cultivar: Balady) showed higher phenolic content (791.98 mg/d.wt) and higher antioxidant activity (93%) than those of the ethanolic extract of A. santolina (340.23 mg/d.wt) and (72.72%), respectively. R. sativus methanolic extract showed lower flavonoids contents (1.025 mg/g d.wt) than A. santolina ethanolic extract (24.66 mg/g d.wt). Treatment of Caco2, HepG2, MCF7 and WISH cell lines with A. santolina extract showed IC50 of 17.67 g/ml, 15.12 g/ml, 42.19 g/ml and 50.99 g/ml, respectively. While treatment of the same cell line with R. sativus showed IC50 of 40.77 g/ml, 27.42 g/ml, 54.16 g/ml and 86.37 g/ml respectively. A. santolina and R. sativus extracts induced similar cell cycle arrest in Caco2 at G1 phase by 42.4%. This study indicates that A. santolina has a potent anticancer activity against the selected cancer cell lines.

Key words: Achillea santolina, Raphanus sativus, Cell lines, cytotoxicity, cell cycle, apoptosis.


 
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How to Cite this Article
Pubmed Style

Salem ML, Gafaar RM, Mohasseb RN, Abd-Elbaseer MA. Apoptotic effects of the medicinal plants Achillea santolina and Raphanus sativus extracts on different cancer cell lines. JCBR. 2018; 1(2): 1-10. doi:104-1503657340


Web Style

Salem ML, Gafaar RM, Mohasseb RN, Abd-Elbaseer MA. Apoptotic effects of the medicinal plants Achillea santolina and Raphanus sativus extracts on different cancer cell lines. http://www.jcbreg.com/?mno=276302 [Access: August 25, 2019]. doi:104-1503657340


AMA (American Medical Association) Style

Salem ML, Gafaar RM, Mohasseb RN, Abd-Elbaseer MA. Apoptotic effects of the medicinal plants Achillea santolina and Raphanus sativus extracts on different cancer cell lines. JCBR. 2018; 1(2): 1-10. doi:104-1503657340



Vancouver/ICMJE Style

Salem ML, Gafaar RM, Mohasseb RN, Abd-Elbaseer MA. Apoptotic effects of the medicinal plants Achillea santolina and Raphanus sativus extracts on different cancer cell lines. JCBR. (2018), [cited August 25, 2019]; 1(2): 1-10. doi:104-1503657340



Harvard Style

Salem, M. L., Gafaar, . R. M., Mohasseb, . R. N. & Abd-Elbaseer, . M. A. (2018) Apoptotic effects of the medicinal plants Achillea santolina and Raphanus sativus extracts on different cancer cell lines. JCBR, 1 (2), 1-10. doi:104-1503657340



Turabian Style

Salem, Mohamed Labib, Reda Mohamed Gafaar, Reham Nagy Mohasseb, and Mohamed Ali Abd-Elbaseer. 2018. Apoptotic effects of the medicinal plants Achillea santolina and Raphanus sativus extracts on different cancer cell lines. Journal of Cancer and Biomedical Research, 1 (2), 1-10. doi:104-1503657340



Chicago Style

Salem, Mohamed Labib, Reda Mohamed Gafaar, Reham Nagy Mohasseb, and Mohamed Ali Abd-Elbaseer. "Apoptotic effects of the medicinal plants Achillea santolina and Raphanus sativus extracts on different cancer cell lines." Journal of Cancer and Biomedical Research 1 (2018), 1-10. doi:104-1503657340



MLA (The Modern Language Association) Style

Salem, Mohamed Labib, Reda Mohamed Gafaar, Reham Nagy Mohasseb, and Mohamed Ali Abd-Elbaseer. "Apoptotic effects of the medicinal plants Achillea santolina and Raphanus sativus extracts on different cancer cell lines." Journal of Cancer and Biomedical Research 1.2 (2018), 1-10. Print. doi:104-1503657340



APA (American Psychological Association) Style

Salem, M. L., Gafaar, . R. M., Mohasseb, . R. N. & Abd-Elbaseer, . M. A. (2018) Apoptotic effects of the medicinal plants Achillea santolina and Raphanus sativus extracts on different cancer cell lines. Journal of Cancer and Biomedical Research, 1 (2), 1-10. doi:104-1503657340