E-ISSN 2537-0596 | ISSN 2537-0588
 

Original Research 


Ex vivo generation and maturation of dendritic cells from peripheral blood mononuclear cells of patients with chronic HCV or hepatocellular carcinoma using Toll-like receptor 3 ligand poly(I:C)

Mohamed L. Salem, Soha Gomaa, Mohamed Nassef, Sabry A. El-Naggar, Abdel-Aziz A. Zidan, Essam M. Ibrahim, Hassan El-Bate, Abdel-Rahman N. Zekri, Hala A. Abdelhady..

Abstract
Chronic hepatitis C virus (HCV) infection and hepatocellular carcinoma (HCC) resulted in dysfunction of the immune response, in particular, dendritic cells (DCs). Stimulation and reactivation of DCs could restore the immune responses in this disease. Our previous study revealed that a synthetic double-stranded RNA (polyI:C) recognized by toll-like receptor 3 (TLR3) induced a potent innate immune response and had an impact on DCs both ex vivo and in vivo. The current study was aimed to generate and maturate DCs from peripheral blood mononuclear cells (PBMCs) of healthy controls and patients with HCV and HCC. Fresh peripheral blood (PB) was collected from healthy controls, patients with chronic HCV or patients with hepatocellular carcinoma (HCC). PBMCs were isolated and cultured for 6 days in RPMI-1640 supplemented with GM-CSF and rhIL-4 (10 ng/mL each). Poly(I:C) was added to the culture on day 6 and the cells were harvested on day 7. Phenotypic analysis of DCs and their maturation markers were assessed using flow cytometry. DCs were generated from adherent PBMCs of healthy donors or patients with HCV or HCC. Interestingly, the addition of poly(I:C) induced expansion and maturation of DCs as evidenced by the expression of HLA-DR and CD11bCD11c surface molecules on the DCs generated from all groups. In conclusion, DCs can be ex vivo generated from control or patients with HCV or HCC in response to external stimulation. These results may be a promising tool for a therapeutic vaccine against HCV infection.

Key words: Poly(I:C), Dendritic cells, HCV, HCC.


 
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How to Cite this Article
Pubmed Style

Salem ML, Gomaa S, Nassef M, El-Naggar SA, Zidan AA, Ibrahim EM, El-Bate H, Zekri AN, Abdelhady. HA. Ex vivo generation and maturation of dendritic cells from peripheral blood mononuclear cells of patients with chronic HCV or hepatocellular carcinoma using Toll-like receptor 3 ligand poly(I:C). JCBR. 2018; 1(1): 11-18. doi:104-1505080675


Web Style

Salem ML, Gomaa S, Nassef M, El-Naggar SA, Zidan AA, Ibrahim EM, El-Bate H, Zekri AN, Abdelhady. HA. Ex vivo generation and maturation of dendritic cells from peripheral blood mononuclear cells of patients with chronic HCV or hepatocellular carcinoma using Toll-like receptor 3 ligand poly(I:C). http://www.jcbreg.com/?mno=277556 [Access: December 12, 2018]. doi:104-1505080675


AMA (American Medical Association) Style

Salem ML, Gomaa S, Nassef M, El-Naggar SA, Zidan AA, Ibrahim EM, El-Bate H, Zekri AN, Abdelhady. HA. Ex vivo generation and maturation of dendritic cells from peripheral blood mononuclear cells of patients with chronic HCV or hepatocellular carcinoma using Toll-like receptor 3 ligand poly(I:C). JCBR. 2018; 1(1): 11-18. doi:104-1505080675



Vancouver/ICMJE Style

Salem ML, Gomaa S, Nassef M, El-Naggar SA, Zidan AA, Ibrahim EM, El-Bate H, Zekri AN, Abdelhady. HA. Ex vivo generation and maturation of dendritic cells from peripheral blood mononuclear cells of patients with chronic HCV or hepatocellular carcinoma using Toll-like receptor 3 ligand poly(I:C). JCBR. (2018), [cited December 12, 2018]; 1(1): 11-18. doi:104-1505080675



Harvard Style

Salem, M. L., Gomaa, . S., Nassef, . M., El-Naggar, . S. A., Zidan, . A. A., Ibrahim, . E. M., El-Bate, . H., Zekri, . A. N. & Abdelhady., . H. A. (2018) Ex vivo generation and maturation of dendritic cells from peripheral blood mononuclear cells of patients with chronic HCV or hepatocellular carcinoma using Toll-like receptor 3 ligand poly(I:C). JCBR, 1 (1), 11-18. doi:104-1505080675



Turabian Style

Salem, Mohamed L., Soha Gomaa, Mohamed Nassef, Sabry A. El-Naggar, Abdel-Aziz A. Zidan, Essam M. Ibrahim, Hassan El-Bate, Abdel-Rahman N. Zekri, and Hala A. Abdelhady.. 2018. Ex vivo generation and maturation of dendritic cells from peripheral blood mononuclear cells of patients with chronic HCV or hepatocellular carcinoma using Toll-like receptor 3 ligand poly(I:C). Journal of Cancer and Biomedical Research, 1 (1), 11-18. doi:104-1505080675



Chicago Style

Salem, Mohamed L., Soha Gomaa, Mohamed Nassef, Sabry A. El-Naggar, Abdel-Aziz A. Zidan, Essam M. Ibrahim, Hassan El-Bate, Abdel-Rahman N. Zekri, and Hala A. Abdelhady.. "Ex vivo generation and maturation of dendritic cells from peripheral blood mononuclear cells of patients with chronic HCV or hepatocellular carcinoma using Toll-like receptor 3 ligand poly(I:C)." Journal of Cancer and Biomedical Research 1 (2018), 11-18. doi:104-1505080675



MLA (The Modern Language Association) Style

Salem, Mohamed L., Soha Gomaa, Mohamed Nassef, Sabry A. El-Naggar, Abdel-Aziz A. Zidan, Essam M. Ibrahim, Hassan El-Bate, Abdel-Rahman N. Zekri, and Hala A. Abdelhady.. "Ex vivo generation and maturation of dendritic cells from peripheral blood mononuclear cells of patients with chronic HCV or hepatocellular carcinoma using Toll-like receptor 3 ligand poly(I:C)." Journal of Cancer and Biomedical Research 1.1 (2018), 11-18. Print. doi:104-1505080675



APA (American Psychological Association) Style

Salem, M. L., Gomaa, . S., Nassef, . M., El-Naggar, . S. A., Zidan, . A. A., Ibrahim, . E. M., El-Bate, . H., Zekri, . A. N. & Abdelhady., . H. A. (2018) Ex vivo generation and maturation of dendritic cells from peripheral blood mononuclear cells of patients with chronic HCV or hepatocellular carcinoma using Toll-like receptor 3 ligand poly(I:C). Journal of Cancer and Biomedical Research, 1 (1), 11-18. doi:104-1505080675