E-ISSN 2537-0596 | ISSN 2537-0588
 

Original Research 


Analysis of Micro RNA 29, 122 and 155 Gene Expression in Immune Cells of Egyptian Patients with Chronic Hepatitis C Virus Infection

mohamed labib salem, abed el aziz awad zidan, hasan el sayed al batei, hossam youssef hamada.

Abstract
Infected patients with chronic hepatitis C virus (HCV) showed high numbers of myeloid-derived suppressor cells (MDSCs) which are known to suppress the immune response. Given that microRNAs regulate the expression of many genes, it is important to test whether MDSCs correlate with altered miRNA expression. Aim: The present study aimed to assess the expression of miR-29, 122 and 155 in chronic HCV patients under interferon (IFN) therapy and their correlation with the frequency of MDCS. A total of 28 subjects, including eighteen chronic HCV patients, and 10 healthy volunteers were enrolled in this study. MiR-29, 122 and 155 were evaluated using real-time- polymerase chain reaction (RT-PCR). Flow cytometric analysis was performed to evaluate the MDSCs which defined as Lin-HLA-DR- CD33+ CD11b+. Results: miR-29 and 122 expressions in HCV patients showed significant decrease when compared to their control. However, there was an increase (but non-significance) in miR-155 in HCV patients than control subjects. Both relative and absolute numbers of MDSCs were increased in the peripheral blood in HCV patients as compared to control subjects. Moreover, non- significance change in IFNα/β receptors was observed in HCV patients as compared to control subjects. Conclusion: High numbers of MDSCs in chronic HCV patients correlate with low expression of miR-29 and122.

Key words: miR:Micro RNA HCV:Hepatits C virus IFN: interferon MDSC:myeloid derived supressor cell


 
ARTICLE TOOLS
Abstract
PDF Fulltext
Print this article Print this Article
How to cite this articleHow to cite this article
Citation Tools
Related Records
 Articles by mohamed labib salem
Articles by abed el aziz awad zidan
Articles by hasan el sayed al batei
Articles by hossam youssef hamada
on Google
on Google Scholar
Article Statistics
 Viewed: 158
Downloaded: 96
Cited: 0

How to Cite this Article
Pubmed Style

salem ml, zidan aeaa, batei hesa, hamada hy. Analysis of Micro RNA 29, 122 and 155 Gene Expression in Immune Cells of Egyptian Patients with Chronic Hepatitis C Virus Infection. JCBR. 2018; 1(1): 37-44.


Web Style

salem ml, zidan aeaa, batei hesa, hamada hy. Analysis of Micro RNA 29, 122 and 155 Gene Expression in Immune Cells of Egyptian Patients with Chronic Hepatitis C Virus Infection. http://www.jcbreg.com/?mno=277689 [Access: December 12, 2018].


AMA (American Medical Association) Style

salem ml, zidan aeaa, batei hesa, hamada hy. Analysis of Micro RNA 29, 122 and 155 Gene Expression in Immune Cells of Egyptian Patients with Chronic Hepatitis C Virus Infection. JCBR. 2018; 1(1): 37-44.



Vancouver/ICMJE Style

salem ml, zidan aeaa, batei hesa, hamada hy. Analysis of Micro RNA 29, 122 and 155 Gene Expression in Immune Cells of Egyptian Patients with Chronic Hepatitis C Virus Infection. JCBR. (2018), [cited December 12, 2018]; 1(1): 37-44.



Harvard Style

salem, m. l., zidan, . a. e. a. a., batei, . h. e. s. a. & hamada, . h. y. (2018) Analysis of Micro RNA 29, 122 and 155 Gene Expression in Immune Cells of Egyptian Patients with Chronic Hepatitis C Virus Infection. JCBR, 1 (1), 37-44.



Turabian Style

salem, mohamed labib, abed el aziz awad zidan, hasan el sayed al batei, and hossam youssef hamada. 2018. Analysis of Micro RNA 29, 122 and 155 Gene Expression in Immune Cells of Egyptian Patients with Chronic Hepatitis C Virus Infection. Journal of Cancer and Biomedical Research, 1 (1), 37-44.



Chicago Style

salem, mohamed labib, abed el aziz awad zidan, hasan el sayed al batei, and hossam youssef hamada. "Analysis of Micro RNA 29, 122 and 155 Gene Expression in Immune Cells of Egyptian Patients with Chronic Hepatitis C Virus Infection." Journal of Cancer and Biomedical Research 1 (2018), 37-44.



MLA (The Modern Language Association) Style

salem, mohamed labib, abed el aziz awad zidan, hasan el sayed al batei, and hossam youssef hamada. "Analysis of Micro RNA 29, 122 and 155 Gene Expression in Immune Cells of Egyptian Patients with Chronic Hepatitis C Virus Infection." Journal of Cancer and Biomedical Research 1.1 (2018), 37-44. Print.



APA (American Psychological Association) Style

salem, m. l., zidan, . a. e. a. a., batei, . h. e. s. a. & hamada, . h. y. (2018) Analysis of Micro RNA 29, 122 and 155 Gene Expression in Immune Cells of Egyptian Patients with Chronic Hepatitis C Virus Infection. Journal of Cancer and Biomedical Research, 1 (1), 37-44.