E-ISSN 2537-0596 | ISSN 2537-0588
 

Original Research 


In vitro anti-tumor effects of hemocyanin isolated from Atergatis roseus and Eriphia verrucosa crabs

Wesam Mohamed Salama, Mahy Mohamed Mona.

Abstract
Hemocyanin (HCY) is a multifunctional glycoprotein, which plays multiple roles in immune defense in invertebrates. HCY from some mollusks can induce potent immune response, while little is currently known about how hemocyanin from arthropods affects tumors. In the present study, in vitro cytotoxic effect of HCY from two crab species Atergatis roseus and Eriphia verrucosa was investigated on two cell lines included hepatocellular carcinoma (HepG-2) and breast cancer (MCF-7). The results showed that HCY exhibited degrees of inhibitory activity against the two cell lines. HCY of A.roseus showed the highest cytotoxicity against HepG-2 cell line(IC50 :7.7 3.1 mg/ml) while the HCY of E.verucossa showed inhibitory effect (IC50 : 9.5 2.6 mg/ml) after 72 h. HCY of A.roseus and E.verucossa showed their maximum cytotoxity IC50 towards MCF-7 at 2.71 1.3 mg/ml and 4.72 1.5 mg/ml, respectively. In Conclusion, We concluded that HCY obtained from A. roseus has strong anti-tumor activity against HepG-2 and MCF-7 than HCY obtained from E. verrucosa.

Key words: anti-tumor activity, crabs, hemocyanin, HepG-2, MCF-7


 
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Pubmed Style

Salama WM, Mona MM, . In vitro anti-tumor effects of hemocyanin isolated from Atergatis roseus and Eriphia verrucosa crabs. JCBR. 2018; 1(1): 28-36.


Web Style

Salama WM, Mona MM, . In vitro anti-tumor effects of hemocyanin isolated from Atergatis roseus and Eriphia verrucosa crabs. http://www.jcbreg.com/?mno=2927 [Access: December 12, 2018].


AMA (American Medical Association) Style

Salama WM, Mona MM, . In vitro anti-tumor effects of hemocyanin isolated from Atergatis roseus and Eriphia verrucosa crabs. JCBR. 2018; 1(1): 28-36.



Vancouver/ICMJE Style

Salama WM, Mona MM, . In vitro anti-tumor effects of hemocyanin isolated from Atergatis roseus and Eriphia verrucosa crabs. JCBR. (2018), [cited December 12, 2018]; 1(1): 28-36.



Harvard Style

Salama, W. M., Mona, . M. M. & (2018) In vitro anti-tumor effects of hemocyanin isolated from Atergatis roseus and Eriphia verrucosa crabs. JCBR, 1 (1), 28-36.



Turabian Style

Salama, Wesam Mohamed, Mahy Mohamed Mona, and . 2018. In vitro anti-tumor effects of hemocyanin isolated from Atergatis roseus and Eriphia verrucosa crabs. Journal of Cancer and Biomedical Research, 1 (1), 28-36.



Chicago Style

Salama, Wesam Mohamed, Mahy Mohamed Mona, and . "In vitro anti-tumor effects of hemocyanin isolated from Atergatis roseus and Eriphia verrucosa crabs." Journal of Cancer and Biomedical Research 1 (2018), 28-36.



MLA (The Modern Language Association) Style

Salama, Wesam Mohamed, Mahy Mohamed Mona, and . "In vitro anti-tumor effects of hemocyanin isolated from Atergatis roseus and Eriphia verrucosa crabs." Journal of Cancer and Biomedical Research 1.1 (2018), 28-36. Print.



APA (American Psychological Association) Style

Salama, W. M., Mona, . M. M. & (2018) In vitro anti-tumor effects of hemocyanin isolated from Atergatis roseus and Eriphia verrucosa crabs. Journal of Cancer and Biomedical Research, 1 (1), 28-36.