E-ISSN 2537-0596 | ISSN 2537-0588
 

Original Research 


Administration of olive oil optimizes the acetic acid induced colitis in CD1 mice

Mohamed Labib Salem, Ehab Moustafa Tousson, Soha Gomaa Ramadan, Dina Abd El-Aziz Ali.

Abstract
Colitis is a chronic disease that affects individuals at different ages and affects the quality of their life. So far, there are three different experimental models for colitis. However, animals in these models die quickly duo to the associates toxicity. The aim of this study is to optimize acetic acid induced acute colitis mouse model with olive oil co-administration. Male albino CD1 mice (n= 91) were randomly divided into 13 groups (n= 7/group). To induce colitis, mice were administrated with different volumes (100 or 200 l) and concentrations (1%, 2% and 4%) of acetic acid by intrarectal (IR) injection either for three consecutive days or every other day for a total of 6 days. Olive oil (1 L) was co-injected with acetic acid. One day later, mice were sacrificed and colon tissues were fixed in 10% formalin for histological examinations. The induction of colitis by acetic acid was dependent on both the volume and the concentration of acetic acid. Interestingly, co-administration of olive oil with different volumes and concentrations of acetic acid, although did not alter the severity of inflammation, it increased the animal survival rate and decreased the associated toxicity. Injection of co-administration of olive oil with acetic acid is a new modification to enhance the application of this model.

Key words: Experimental model, Optimization, Colitis, Induction, Acetic acid, Olive oil.


 
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How to Cite this Article
Pubmed Style

Salem ML, Tousson EM, Ramadan SG, Ali DAE. Administration of olive oil optimizes the acetic acid induced colitis in CD1 mice. JCBR. 2018; 1(2): 11-18.


Web Style

Salem ML, Tousson EM, Ramadan SG, Ali DAE. Administration of olive oil optimizes the acetic acid induced colitis in CD1 mice. http://www.jcbreg.com/?mno=297526 [Access: November 12, 2019].


AMA (American Medical Association) Style

Salem ML, Tousson EM, Ramadan SG, Ali DAE. Administration of olive oil optimizes the acetic acid induced colitis in CD1 mice. JCBR. 2018; 1(2): 11-18.



Vancouver/ICMJE Style

Salem ML, Tousson EM, Ramadan SG, Ali DAE. Administration of olive oil optimizes the acetic acid induced colitis in CD1 mice. JCBR. (2018), [cited November 12, 2019]; 1(2): 11-18.



Harvard Style

Salem, M. L., Tousson, . E. M., Ramadan, . S. G. & Ali, . D. A. E. (2018) Administration of olive oil optimizes the acetic acid induced colitis in CD1 mice. JCBR, 1 (2), 11-18.



Turabian Style

Salem, Mohamed Labib, Ehab Moustafa Tousson, Soha Gomaa Ramadan, and Dina Abd El-Aziz Ali. 2018. Administration of olive oil optimizes the acetic acid induced colitis in CD1 mice. Journal of Cancer and Biomedical Research, 1 (2), 11-18.



Chicago Style

Salem, Mohamed Labib, Ehab Moustafa Tousson, Soha Gomaa Ramadan, and Dina Abd El-Aziz Ali. "Administration of olive oil optimizes the acetic acid induced colitis in CD1 mice." Journal of Cancer and Biomedical Research 1 (2018), 11-18.



MLA (The Modern Language Association) Style

Salem, Mohamed Labib, Ehab Moustafa Tousson, Soha Gomaa Ramadan, and Dina Abd El-Aziz Ali. "Administration of olive oil optimizes the acetic acid induced colitis in CD1 mice." Journal of Cancer and Biomedical Research 1.2 (2018), 11-18. Print.



APA (American Psychological Association) Style

Salem, M. L., Tousson, . E. M., Ramadan, . S. G. & Ali, . D. A. E. (2018) Administration of olive oil optimizes the acetic acid induced colitis in CD1 mice. Journal of Cancer and Biomedical Research, 1 (2), 11-18.