E-ISSN 2537-0596 | ISSN 2537-0588
 

Original Research 


Metabolic changes in nave and activated T-cell from naive mice

Mohammed Labib Salem, Tarek Mostafa Mohammed, Mona Mohammed Hegazi, Asmaa Abd Elsamad Eltaramsy.

Abstract
We have found previously that early activated T cells show higher activities and antitumor effects than late activated T cells upon their adoptive transfer. However, the exact mechanism is not clear. This study aimed to investigate the impact of polyclonal activation on the metabolic signature of T cells from nave mice. Splenocytes of Swiss albino mice were harvested and cultured at 2.5 106/ml in T25 flasks in presence of (Con-A) and IL2 for 24h or 72h Then, cells were harvested and processed to determine activities of hexokinase (HK), phosphofructokinase (PFK), lactate dehydrogenase (LDH) and glucose 6 phosphate dehydrogenase(G6PD). The obtained data showed that activity of enzymes in activated cellswas increased after 24h and 72h activation as compared to nave cells, when and supernatant their level at 72h activation was higher than 24h activation. Comparison of enzymes activity between cells and supernatant showed higher activity of HK, G6PD and LDH in cells, While PFK activity was higher in supernatant after 72h.these results indicate that Upon the early activation, nave T cells have higher glycolytic pathway than late activation. These results have a great implication in adoptive T cell therapy

Key words: Metabolic changes, nave, T cells.


 
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How to Cite this Article
Pubmed Style

Salem ML, Mohammed TM, Hegazi MM, Eltaramsy AAE, . Metabolic changes in nave and activated T-cell from naive mice. JCBR. 2018; 1(2): 19-25.


Web Style

Salem ML, Mohammed TM, Hegazi MM, Eltaramsy AAE, . Metabolic changes in nave and activated T-cell from naive mice. http://www.jcbreg.com/?mno=702 [Access: August 25, 2019].


AMA (American Medical Association) Style

Salem ML, Mohammed TM, Hegazi MM, Eltaramsy AAE, . Metabolic changes in nave and activated T-cell from naive mice. JCBR. 2018; 1(2): 19-25.



Vancouver/ICMJE Style

Salem ML, Mohammed TM, Hegazi MM, Eltaramsy AAE, . Metabolic changes in nave and activated T-cell from naive mice. JCBR. (2018), [cited August 25, 2019]; 1(2): 19-25.



Harvard Style

Salem, M. L., Mohammed, . T. M., Hegazi, . M. M., Eltaramsy, . A. A. E. & (2018) Metabolic changes in nave and activated T-cell from naive mice. JCBR, 1 (2), 19-25.



Turabian Style

Salem, Mohammed Labib, Tarek Mostafa Mohammed, Mona Mohammed Hegazi, Asmaa Abd Elsamad Eltaramsy, and . 2018. Metabolic changes in nave and activated T-cell from naive mice. Journal of Cancer and Biomedical Research, 1 (2), 19-25.



Chicago Style

Salem, Mohammed Labib, Tarek Mostafa Mohammed, Mona Mohammed Hegazi, Asmaa Abd Elsamad Eltaramsy, and . "Metabolic changes in nave and activated T-cell from naive mice." Journal of Cancer and Biomedical Research 1 (2018), 19-25.



MLA (The Modern Language Association) Style

Salem, Mohammed Labib, Tarek Mostafa Mohammed, Mona Mohammed Hegazi, Asmaa Abd Elsamad Eltaramsy, and . "Metabolic changes in nave and activated T-cell from naive mice." Journal of Cancer and Biomedical Research 1.2 (2018), 19-25. Print.



APA (American Psychological Association) Style

Salem, M. L., Mohammed, . T. M., Hegazi, . M. M., Eltaramsy, . A. A. E. & (2018) Metabolic changes in nave and activated T-cell from naive mice. Journal of Cancer and Biomedical Research, 1 (2), 19-25.